Representative sorting results of CD4⁺CD25⁺CCR4⁺ cells. After labeling with anti-CD3-FITC, anti-CD4-Cy7, anti-CD25-APC, and anti-CCR4-PE, the CD3⁺CD4⁺CD25⁺CCR4⁺ fraction was isolated. The averaged content ± standard deviation (%) of CD25⁺CCR4⁺ cells out of CD3⁺CD4⁺ cells was calculated for each clinical group and is displayed in the upper right section of the panels.