Gene expression analyses show alterations in CD41-KO HSCs and suggest differential lineage priming of CD41+ WT HSCs compared with CD41− WT HSCs. (A) GSEA plots showing top gene sets downregulated in CD41-KO LSKCD150+CD48− LT-HSCs compared with WT. (B) Heat map showing the expression of selected genes differentially expressed in CD41+ and CD41− WT LSKflt3− HSCs and mentioned in the text. Black/white indicates enrichment/depletion. (C) FACS plots showing the coexpression of TGFβR3 within a subset of CD41+, but not CD41−, LT-HSC (LSKCD48−CD150+), or ST-HSC+LMPP (LSKCD48+CD150−) fractions. (D) GSEA enrichment plots for transcription factor motifs enriched in CD41+ (left) and CD41− (right) HSCs.