Effect of U0126 on the expression of CCL2 and genes involved in LDL binding in immature MDDCs. (A) Relative expression of the indicated genes in immature DCs treated with DMSO (-) or U0126 (UO) for 24 hours. Results are expressed as relative expression, which indicates the expression of each gene relative to its expression in DMSO-treated MDDCs (*P < .05). Shown are the means and SDs of three independent experiments. (B) LDL binding to immature DCs exposed to either DMSO or U0126 for 24 hours and exposed to LDL for the indicated periods of time, as determined by flow cytometry. The lower panel illustrates the percentage of LDL-positive cells in each sample (*P < .05). The means and SDs of five independent experiments are presented. (C) Relative expression of the CCL2 gene in immature DCs treated with DMSO (-), U0126 (UO), or PD98059 (PD) after the indicated time of treatment. The means and SDs of three independent experiments for UO and two experiments for PD-treated MDDCs are presented. Results are expressed as relative expression, which indicates CCL2 expression in each sample relative to its expression in DMSO-treated MDDCs (*P < .05). (D) Expression of CCL2 (MCP1) in immature DCs treated with either DMSO (-), U0126 (UO), or PD98059 (PD) after the indicated time of treatment, as determined by enzyme-linked immunosorbent assay. Shown are the means and SDs of four independent experiments (*P < .05).