Effect of U0126 on the expression of DC maturation-associated genes. (A) Relative expression of the indicated genes in LPS-treated mature MDDCs. Results are expressed as relative expression (log scale), which indicates the expression of each gene in mature MDDCs relative to its expression in immature MDDCs. (B) Relative expression of the indicated genes in immature DCs from four different donors treated with DMSO (-) or U0126 (UO) for 24 hours. Results are expressed as relative expression, which indicates the expression of each gene relative to its expression in DMSO-treated MDDCs (*P < .05). (C) CCL19-directed migration of immature DCs exposed to either DMSO (-), U0126 (UO), or DMSO + LPS (LPS) for 24 hours, as determined in Transwell migration assays on immature DCs from two independent donors. Results are expressed as percentage of migrated cells relative to the input in the presence and absence of CCL19.