Determination of the U0126-dependent transcriptomic profile of immature MDDCs. (A) Schematic representation of the profiling experiments, with indication of the treatment and time points for sample collection. (B) Effect of U0126 (UO) or DMSO (-) treatment on ERK1/2 phosphorylation in immature MDDCs, as determined by western blot. The level of total ERK1/2 and GAPDH protein were determined in parallel. The histogram illustrates the ratio p-ERK/ERK for each sample, as determined by densitometric analysis of the western blot. One of 3 representative experiments is presented. (C) Quantification of the number of genes whose expression is significantly altered (adjusted P < .05) at all time points (Total) and after the indicated duration of treatment with U0126. (D) Comparison of the number of genes whose expression is significantly (adjusted P < .05) upregulated (left panel) or downregulated (right panel) after the indicated time of exposure to U0126. (E) GO analysis for molecular functions significantly (adjusted P < .05) enriched within the sets of genes whose expression is altered by U0126 exposure at the indicated times. (F) Number of genes whose expression is significantly (adjusted P < .05) upregulated or downmodulated by U0126 at the indicated times and for the indicated GO terms.