SF3B1 mutation is a predominantly subclonal event in CLL. (A) Percentage of the mutations classified as clonal (orange) and subclonal (blue) for each putative CLL driver, within a cohort of 149 CLL cases. The number of cases (n) affected by each genetic alteration is shown (*Drivers with q value < 0.1 for a higher proportion of clonal mutations compared with the entire CLL drivers set). (B) Analysis of co-occurrence of the 19 SF3B1 mutations within 149 CLL cases with other driver alterations. Top bar shows the color representation of CCF. (C) Joint distributions of CCF values across 2 time points using clustering analysis (see Landau et al for method19 ). Red denotes a mutation that had an increase in CCF of >0.2 (with probability >0.5). The dotted diagonal line represents CCF values that were identical across the 2 time points. The dotted parallel lines denote the 0.2 CCF interval on either side. Panel A adapted from Landau et al19 with permission.
