Figure 3
![Figure 3. A ZFN chromosomal translocation assay. (A) Schematic of the ZFN translocation assay in HEK 293T cells, where simultaneous DSBs are induced in chromosomes 1 and 3, then nested semiquantitative PCR is used to identify the der[3] translocation product. A ZFN pair is required to nick both DNA strands for DSB generation. (B) In vitro assay showing that PARP1 poly(ADP-ribosyl)ation activity is inhibited up to 3.3-fold by olaparib in HEK 293T cells in the presence or absence of ZFNs. (C) siRNA against PARP1 repressed PARP1 protein expression. (D) PARP1 inhibition with olaparib does not decrease ZFN expression. (E) PARP1 siRNA does not decrease ZFN expression.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/21/10.1182_blood-2012-10-460527/4/4359f3.jpeg?Expires=1724956858&Signature=LFAl7F3-1etNopAJCssMheXv97~7NYhPkuDi50-kdibjAfOAKWKh2~koDRGxR2iwDPcqVA5XUYu9ua8FwWTQqMOGXkDAzaT3iEsU-OmpZz0OstJ9SgwoAYKu2Snq0RhwtEGBcXhh9iNuZ~uGE0EaQItpA57cUUJeufvO3ttkmwCc2sEoxQ~pQ6-CRVge3dgZiGLm7VR8PqYkgqIpDiaGchvdcsu3UrZT~53cby7hgEIuBhABqMdM3EciCLbmf9xZTxeeIm-q9BXqZARG0nNlXw26ZRLhsE2j6MgF3DALj1Rpl8~jW7taNIwOkdOTITuESqftdfV70BambZ9BJwzy9A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
A ZFN chromosomal translocation assay. (A) Schematic of the ZFN translocation assay in HEK 293T cells, where simultaneous DSBs are induced in chromosomes 1 and 3, then nested semiquantitative PCR is used to identify the der[3] translocation product. A ZFN pair is required to nick both DNA strands for DSB generation. (B) In vitro assay showing that PARP1 poly(ADP-ribosyl)ation activity is inhibited up to 3.3-fold by olaparib in HEK 293T cells in the presence or absence of ZFNs. (C) siRNA against PARP1 repressed PARP1 protein expression. (D) PARP1 inhibition with olaparib does not decrease ZFN expression. (E) PARP1 siRNA does not decrease ZFN expression.
