The NOG mouse model can support self-renewal of TAM-derived cells. (A) May-Giemsa staining of TAM-derived cells in recipients of patient 1. (B) Surface marker analysis of TAM-derived cells in recipients by flow cytometry. Viable cells were gated according to their forward scatter (FSC) and 4′,6-diamidino-2-phenylindole staining, blast cells were identified by CD45/SSC gating, and hCD45⁺-gated cells were tested for the expression of hCD117, hCD34, hCD33, and hCD41a. (C) Proportion of hCD45⁺ cells in BM of 1°, 2°, and 3° recipient mice at 4, 8, and 12 weeks after transplantation. (D) Colony assay of hCD45⁺ cells in BM of 1°, 2°, and 3° recipient mice. hCD45⁺ cells were seeded at 1.0 × 10⁴ cells per 35-mm dish in triplicate, and the number of colonies in each dish was counted. Bars represent the standard deviation of the mean of 3 independent experiments. *Significant difference (P < .05).