Replication timing profile of eNOS and EC-enriched genes in ECs and non-ECs. (A) A schematic of the NOS3 locus, with primer locations used to detect eNOS. The relative abundance (percent IP) of (B) the proximal promoter of eNOS and (C) exon 14 of eNOS in each cell cycle fraction quantified by qPCR in HUVECs (gray) and HuAoVSMCs (black). Data represent mean ± standard error of the mean, n = 4. The relative abundance (percent IP) of EC-enriched genes in each cell cycle fraction is shown for (D) CD31, (E) ICAM-2, (F) VEGFR-1, and (G) VEGFR-2, quantified by qPCR. Data represent mean ± standard error of the mean, n = 3.