CNS infiltration by lymphoma cells in mice receiving syngeneic anti–third-party Tcm. Lethally irradiated (8Gy TBI) BALB/c mice (H-2^d) received an intravenous transplant of 3 × 10⁶ syngeneic TDBM cells (BALB/c nude [H-2^d]) in the presence 5 × 10³A20-luc lymphoma cells (day 0). Mice were then intravenously injected with 1 × 10⁷ BALB/c-derived (syngeneic) anti–third-party Tcm (A20+Tcm; n = 6). Mice suffering from hindlimb paralysis were monitored by BLI (upper panel, anterior view; middle panel, posterior view). The mice were then killed and brain, spinal cord, cranium, and spinal column were dissected and analyzed by BLI (lower panel).