Quantitative real-time reverse transcription-PCR of adhesion relevant genes. Differential expression of (A) Selpl encoding for PSGL-1 and (B) Il8rb encoding for CXCR2 in neutrophils obtained from E13 fetuses, E18 fetuses, newborn mice, and adult mice. Real-time PCR was performed using neutrophil cDNA reversely transcribed from mRNA with the help of primers specific for adhesion relevant genes as indicated. Gyc and B2M (housekeeping genes) were used to create ΔCT values of adhesion relevant genes tested. Gene expression in adult mice is set to 1 (2−ΔΔCT method), and x-fold expression is shown for all other time points. Values represent mean ± standard error of the mean from ≥3 independent experiments per group. Significant differences to adult values are indicated by the asterisk.