Figure 4
![Figure 4. Microflow chamber experiments. Isolated EGFP+ cells from E13 to E18 fetuses and microflow chambers (20 × 200-µm rectangular capillaries) coated with either (A) rmP-selectin, rmICAM-1, and rmCXCL1 or with (B) rmE-selectin, rmICAM-1, and rmCXCL1 were used to observe adhesion of EGFP+ cells (adherent cells per field of view [FOV], mean ± standard error of the mean from n > 3 mice per group) in the flow chamber at a wall shear stress of 1 dyne/cm2. (C) Adhesion of adult neutrophils in flow chambers coated with P-selectin or E-selectin or buffer alone. Significant differences (P < .05) in rolling to E18 are indicated by the asterisk.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/21/10.1182_blood-2012-07-447144/4/e118f4.jpeg?Expires=1724965326&Signature=PxW9ysGIu2eipaVqebpNjCZGxUPyjrMORuxqP5LstKimiIfNi3K6pY-qDaFuBlMOfwYjMAPVel8Shge9g8rwHv0RxpMItDenuYG6d2tnh0CRolkRsEpjOsS5eYFSTN0-Eoc~TYOqo6R-ipLVC3bmUaxzqz-cP2PDiuDqevVOvHJaJtL9VQFXPmxPeK9O-xzFY0J3pa1Og4nJm9qca16W4cFDIVfYsy0h5NsDxz~pytZTzt1x6Nfj4kryg~gaU0OTGh8k7cgbMk-lsOew~1fTTfhjDsb1Ye7GQEwr8DStinIDGDfaDieGzqrn-4Ci91PYH91etKqDWeC2d92MZCLg4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Microflow chamber experiments. Isolated EGFP+ cells from E13 to E18 fetuses and microflow chambers (20 × 200-µm rectangular capillaries) coated with either (A) rmP-selectin, rmICAM-1, and rmCXCL1 or with (B) rmE-selectin, rmICAM-1, and rmCXCL1 were used to observe adhesion of EGFP+ cells (adherent cells per field of view [FOV], mean ± standard error of the mean from n > 3 mice per group) in the flow chamber at a wall shear stress of 1 dyne/cm2. (C) Adhesion of adult neutrophils in flow chambers coated with P-selectin or E-selectin or buffer alone. Significant differences (P < .05) in rolling to E18 are indicated by the asterisk.
