Figure 2
![Figure 2. IKK enzyme is active and the upstream signaling CBM complex is present in platelets. (A) Mouse and human platelets were held resting (R) or stimulated with thrombin (0.1 U/mL, 3 minutes; S) and extracts from them were subjected to immunoblotting with anti-CARMA1, anti-MALT1, anti-Bcl10, anti-IKK-α, anti-IKK-β, and anti-IKK-γ/NEMO antibodies. (B-C) Mouse platelets were preincubated with IKK inhibitors (BMS-345541, 5 μM; TPCA-1, 0.5 μM [B]) or BAY 11-7082, 12.5 μM; [C]) for 5 minutes and stimulated with thrombin prior to immunoblotting the extracts with anti-pSer32-IκB (pIκB) or anti-IκB antibodies.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/22/10.1182_blood-2012-11-470468/5/4567f2.jpeg?Expires=1722709693&Signature=KmHSmdrxlcQb24YHBR7Jx06vr51lgc~15mvyUqXEJGEncbwU7ClVnARiiCej44R3~9rh7Ed5bhSqQKZ-gd6ObVrzpbVW4zflC-bTaCWg8kFgjLrl9v76XvvYF01ECS6QliEkKuiznvxjXXEVzB03giS7LTRmK90rRNCv8k8~0sAg7EsJTEPBJimBkiQka3T6MR3~J9csBkplBrl8UC55-eSL8K1jr1wgCfKNaRXmBQo-IdaGHak30ATLvxSegnIRdtFCZb~LltCgYt0EFhcAG7dSI8j5o-~oI3Yrf6tdC8awBN6NSsc32CSWdEnxkV4erE8bBGJGY07aCcfajKvl-g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IKK enzyme is active and the upstream signaling CBM complex is present in platelets. (A) Mouse and human platelets were held resting (R) or stimulated with thrombin (0.1 U/mL, 3 minutes; S) and extracts from them were subjected to immunoblotting with anti-CARMA1, anti-MALT1, anti-Bcl10, anti-IKK-α, anti-IKK-β, and anti-IKK-γ/NEMO antibodies. (B-C) Mouse platelets were preincubated with IKK inhibitors (BMS-345541, 5 μM; TPCA-1, 0.5 μM [B]) or BAY 11-7082, 12.5 μM; [C]) for 5 minutes and stimulated with thrombin prior to immunoblotting the extracts with anti-pSer32-IκB (pIκB) or anti-IκB antibodies.
