HLA-DRB1*11 donors only present ADAMTS13 CUB domain peptides. DCs from donors 3 and 4 were incubated with 100 nM or 500 nM of ADAMTS13. MHC II–peptide complexes were purified and the eluted peptides were identified using mass spectrometry and Proteome Discoverer version 1.2. (A) Representation of peptides obtained for all 4 samples. (B-C) Total ion current chromatogram of peptides recovered from cells pulsed with 100 nM (blue) and 500 nM (red) ADAMTS13 for donors 3 and 4. Reconstructed ion chromatogram of GCRLFINVAPRIA peptide obtained from cells pulsed with 100 nM (blue) and 500 nM (red) ADAMTS13 for donors 3 and 4.