Figure 3
![Figure 3. PRT/H Abs activate platelets in a protamine-dependent manner. Washed 14C-serotonin–labeled platelets from healthy donors were incubated with or without protamine (PRT; 1.25 ug/mL) in the presence of 3 representative patient samples obtained at day 30 (CPB patients or control patients [CTRL]) with increasing amounts of unfractionated heparin (0, 0.25, 1, 100 U or 1U+ IV.3). 14C releasate was measured by liquid scintillation counting. These results are representative of 4 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/15/10.1182_blood-2012-11-469130/4/2828f3.jpeg?Expires=1724260422&Signature=s6AQWwUx5ekSfiB2jg-825fiSet5lATtfZ6Jp9KxjT-GwpOxb6kcG0Rb2i~qrmN4J~rAQAMqo92x2k5RCHUjib1JMoGdrf~uDTirdLjHUmg56VbmIb7DM8v7FzOkzCKl5y2uXiqOT70I9yWLbmJVVDBL~8W~tJf5Xt4jGxuwnkySp5v-Mjygtn3Z96GUoYPWK1ijPpKMWwIMEwF~ijwREBtqEzZSRcX5tvMX8PGzolip04N0gT7ZYqwWEgeW~hCJVqCjkNbZO3XZ884sL4YvVrofjh~1-8oVWgkvp4zwgT9IRykXLnUGxtKjC9pmsmFOmnoxSxjWIqxWC~rhUjAwew__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PRT/H Abs activate platelets in a protamine-dependent manner. Washed 14C-serotonin–labeled platelets from healthy donors were incubated with or without protamine (PRT; 1.25 ug/mL) in the presence of 3 representative patient samples obtained at day 30 (CPB patients or control patients [CTRL]) with increasing amounts of unfractionated heparin (0, 0.25, 1, 100 U or 1U+ IV.3). 14C releasate was measured by liquid scintillation counting. These results are representative of 4 independent experiments.
