IL-7 administration transiently increases the genetic diversity of the HIV reservoir. (A) Neighbor-joining trees derived from HIV sequences obtained from 4 virally suppressed subjects who received IL-7. CD4⁺ T cells from samples collected at baseline (green dots) and after 4 (blue dots) and 28 days (red dots) following IL-7 therapy were sorted, and a minimum of 20 clones were sequenced in each case. The numbers near nodes indicate the percentage of bootstrap replicates. The scale refers to the distance between sequences. (B) HIV genetic diversities at baseline and 4 and 28 days after IL-7 administration. Each dot represents the mean genetic distance between one given clone and the entire population. The horizontal bar represents the mean and is reflecting the genetic diversity of the viral population at the different time points.