Forms of CD148 and CD45 with short ectodomains abrogate TCR proximal signaling. (A) Immunoblot of phosphorylated LAT (pLAT) and β-actin in B3Z T-cells transduced with either full-length CD148 or truncated CD148 and stimulated with anti-mouse CD3ε coated beads for 15 minutes. (B) Immunoblot of phosphorylated Zap-70 (pZap-70) and β-actin in 2B4 T cells transduced with the indicated CD45 constructs (or vector as control) and stimulated with anti-mouse CD3ε coated beads for 5 minutes. All immunoblots are representative of 3 replicates. The densitometry ratio between either pLAT or pZAP-70 and β-actin was calculated in ImageJ (National Institutes of Health).