In vivo sizes of HSPC compartments and development of granulocyte/monocytes is altered in TgPKR transgenic and PKR knockout mice. (A) BM from 3- to 5-month-old mice was stained for lineage markers Lin, Sca-1, c-Kit, CD34, and CD16/32. The CD34 and CD16/32 staining of the Lin⁻, c-Kit⁺ subset was used to discriminate between hematopoietic progenitor cell populations. (B) Graph depicting the relative frequency of LSK hematopoietic stem cells in total BM. (C) The frequency of CMP cells in BM. (D) The frequency of MEP cells in total BM. (E) The frequency of GMP cells in total BM. (F) BM was stained for the monocyte marker CD11b and measured by flow cytometry. Graph shows the frequency of CD45⁺CD11b⁺ monocytes in total BM. (G) Graph of the average relative frequency of granulocytes (CD45⁺Gr-1⁺) in total BM cells. For all graphs the average data from 5 mice of each genotype is represented.