Transgenic expression of PKR induces BM dysplasia in TgPKR mice. (A and B) Hematoxylin and eosin (H&E) staining of paraffin-embedded sections of femur (for BM) from WT control and TgPKR mice as indicated. White arrows point to normal megakaryocytes. Green arrows indicate dysplastic megakaryocytes with single nuclear lobe or with multiple separated nuclear lobes. (C and D) Wright-Giemsa staining of BM smears from WT control or TgPKR mice as indicated. White arrows indicate normal megakaryocytes. Green arrow indicates a dysplastic megakaryocyte with multiple separated nuclear lobes. (E) Graph showing percentage of dysplastic megakaryocytes in 3- vs 10-month-old WT or TgPKR mice. Five mice of each genotype were analyzed with 200 megakaryocytes counted per specimen.