Flow cytometry analysis of blood and fetal liver cells at E18.5. (A) Gating strategy of Ter119 and CD71-stained blood and fetal liver cells of E18.5 embryos. Mature erythrocytes are in the lower quadrant (CD71⁻Ter119⁺⁾. (B) The percentage of CD71⁻Ter119⁺ cells in E18.5 blood. (C) The percentage of CD71⁻Ter119⁺ cells in E18.5 fetal livers. *P < .05 between mutant and control groups.