Platelet and fibrin accumulation following laser-induced arteriole injury in HA mice upon infusion of FVIII variant proteins. The graphs show the time course of real-time thrombus formation in mice injected with recombinant protein FVIII-BDD (gray) or FVIII-RH (black) with a total of 10 to 15 injuries (n = 3 mice per FVIII variant) for the low-dose cohort and 25 injuries (n = 5 mice per FVIII variant) for the high-dose cohort. Median fluorescence intensity (MFI) for platelet (A) and fibrin (B) are plotted vs time.