G-band karyotype analysis and DNA fingerprint analysis of iPSCs. (A) Cytogenetic analysis of a representative nonmobilized CD34⁺-cell–derived iPSC line after Cre-mediated excision of STEMCCA-loxP, demonstrating a normal karyotype. (B) The identity and origin of that iPSC line is confirmed by DNA microsatellite analysis at 7 loci in comparison with PBMCs derived from that patient. DNA samples were PCR amplified using the Applied Biosystems AmpF? STR COfiler kit and the PCR products were analyzed with an ABI 3130XL Genetic Analyzer using GeneMapper software.