Macrophage Flt1 and CNB1 in wound repair. (A) Quantification of ARA vessels in Flt1 control and mutant aortas. (B,C) Immunolabeling for Iba1 (wound macrophages) and Flt1 in control (Wls^fl/fl) and mutant (Wls^fl/fl;cfms-icre) wound sections (B) and quantification of Flt1 staining intensity (C) (×100). (D) Images of wounds in Flt1 control and mutant animals 5 days after initial injury (×20). (E) Quantification of wound size in Flt1 control and mutant animals. (F) Quantitative reverse-transcription polymerase chain reaction for soluble Flt1 from EOC-2 cells exposed for 24 hours to the factors indicated. (G) Time course of wound repair in animals deficient in CNB1. Statistical analysis was performed using one-way analysis of variance with Tukey’s post-hoc test (A, E, F) and Student t test (C, G) using SPSS (IBM, Armonk, NY) software.