ELISA competition matrix and overlap pattern of anti-A2 mAbs. (A) Competition matrix compiling the results of competition ELISA among 29 anti-A2 mAbs. Rows represent primary mAbs and columns represent biotinylated secondary mAbs. Yellow and white squares represent binding and no binding (no overlap and overlap), respectively, of secondary mAbs. (B) Venn diagram representing overlaps produced by the competition matrix. Each mAb was assigned a group based on its overlap with a basis set of 5 mAbs 4A4—2-93, G48, 2-54, and 1D4—that are underlined and italicized. Colors depict the 8 groups of mAbs defined by their overlap with basis set mAbs: dark red, group A; red, group AB; orange, group B; yellow, group C; green, group BCD; light blue, group D; blue, group DE; and purple, group E.