cNK and ILC22 cells are generated with different cytokine dependency. (A) Day 42 phenotype of CD34⁺-derived cNK (IL-15 and FLT3L) and ILC22 (IL-7, SCF, and FLT3). (B) Ratio of RORC2:Eomes and Ahr:Eomes (measured by real-time qPCR) in the various culture conditions at days 35 and 42. ILC22 cells (generated in IL-7, SCF, and FLT3L) expressed high ratios of RORγt and AhR to Eomes, whereas in contrast cells cultured in only IL-15 and FLT3L showed inverted RORγt:Eomes and AhR:Eomes ratios (n = 3). d, day; FL, FLT3L.