EGFL7 increases EC motility on fibronectin. (A) Random migration of HUVECs seeded on fibronectin, EGFL7, fibronectin mixed with BSA, or fibronectin mixed with EGFL7 was followed using live microscopy. Tracking of individual cells was performed using ImageJ software and data are represented as mean cell velocity (mean ± SEM, n ≥ 3, *P < .05). The red paths in the diagram depict the cells with the lower velocity as compared with an arbitrarily chosen reference value and the black ones represent cells with a higher velocity as compared with the reference. (B) HUVECs were transfected with scrambled siRNA or siRNA targeting β₃ integrin before seeding on fibronectin-coated or fibronectin plus EGFL7–coated slides. Tracking of individual cells was performed as described above. Fn, fibronectin.