American Society of Hematology

Figure 4

$Figure 4. UTX expression is essential for embryonic development. (A) Schematic representation of the UTX conditional allele (UTXFD) and the conversion to the frameshifted KO allele (UTXFDC) after excision of exon 3 with Cre recombinase. (B) Western blot of littermate lysates from one UTXFDC/Y × UTXFDC/wt intercross showed efficient knockout of UTX. (C) UTX KO had no influence on the global level of the tri-methylated histone 3 lysine 27 residue (H3K27me3) relative to the histone 3 (H3) level in embryos derived from an UTXFDC/Y × UTXFDC/wt intercross. (D) Embryos isolated at different stages from UTXFDC/Y × UTXFDC/wt intercrosses. At E9.5 and E10.5, female UTXFDC/FDC embryos show growth retardation, cardiac malformations (arrowhead), and neural tube closure defects (arrow) and die between E11.5 and E13.5. A fraction of UTXFDC/Y male embryos exhibit cranioschisis at all analyzed stages. Scale bar, 1 mm.$

UTX expression is essential for embryonic development. (A) Schematic representation of the UTX conditional allele (UTX^FD) and the conversion to the frameshifted KO allele (UTX^FDC) after excision of exon 3 with Cre recombinase. (B) Western blot of littermate lysates from one UTX^FDC/Y × UTX^FDC/wt intercross showed efficient knockout of UTX. (C) UTX KO had no influence on the global level of the tri-methylated histone 3 lysine 27 residue (H3K27me3) relative to the histone 3 (H3) level in embryos derived from an UTX^FDC/Y × UTX^FDC/wt intercross. (D) Embryos isolated at different stages from UTX^FDC/Y × UTX^FDC/wt intercrosses. At E9.5 and E10.5, female UTX^FDC/FDC embryos show growth retardation, cardiac malformations (arrowhead), and neural tube closure defects (arrow) and die between E11.5 and E13.5. A fraction of UTX^FDC/Y male embryos exhibit cranioschisis at all analyzed stages. Scale bar, 1 mm.

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