Phenotype of KSHV latency locus transgenic mice. Splenocytes were subject to flow cytometric analyses. (A) Representative mature B-cell profiles from wild-type (WT), KSHV LANA transgenic (LANA), and KSHV latency locus transgenic mouse (Latency). Percentages of IgD⁺IgM⁺ lymphocytes among whole lymphocytes are indicated above each gate. (B) Profiles of activated (FSChi) mature B cells are shown. Gates represent CD19⁺IgD⁺IgM⁺FSChi-activated B cells. (C) MZ B cells are gated as IgD^-IgM⁺. (D) Activated MZ B cell (CD19⁺IgD^-IgM⁺FSChi) profiles are shown. (E) Comparison of activated mature B cells defined as CD19⁺IgD⁺IgM⁺FSChi from WT (n = 9), ko (CD19 knockout, n = 4), LANA (KSHV LANA transgenic, n = 13), and latency (KSHV latency locus transgenic, n = 23) mice. tg125 (n = 8) and tg151 (n = 5) mean 2 independent lines of the KSHV LANA transgenic mice. tg442 (n = 9), tg455 (n = 6), tg456 (n = 5), and tg633 (n = 3) represent 4 independent lines of the KSHV latency locus transgenic mice.