GPC3 is expressed on the cell surface of murine as well as human HSPCs. (A) Expression of TF, GPC3, and CD26 on KLS cells. Lin⁻c-kit⁺ BM MNCs were gated (top left) and the cell-surface expression of TF (top right) and GPC3 (bottom left) was determined in combination with Sca-1. GPC3-expressing KLS cells were further gated to determine CD26 expression (bottom right). (B) Freshly isolated MNCs from human UCB samples were analyzed for coexpression of CD26 (top right) and GPC3 (bottom right) in lin⁻CD34⁺ cells (top left) gated for CD38⁻ cells (bottom left). (C) qRT-PCR analysis of Gpc3 expression in different subpopulations of mouse BM (n = 4). (D) qRT-PCR analysis of Gpc3 expression in different subpopulations human UCB-derived MNCs (n = 5). Error bars represent SEM; *P < .05.