Differential expression of migration-related genes upon lenalidomide treatment. A total of 1 × 10⁷ PBMC from CLL patients were cultured in 400 µL complete medium in 24-well plates in the presence or absence of 10 µM lenalidomide for 3 days. Thereafter, CLL cells were enriched by CD19-specific MACS. Gene expression of MMP9, IRF1, RHOC, and CORO1B was analyzed by quantitative reverse-transcription PCR. Results of 4 independently analyzed patient samples were normalized to the expression of 2 housekeeping genes (PGK1, DCTN2) and are depicted as box plots. Whiskers depict 10th and 90th percentile of data. Paired Student t test was performed. CORO1B was significantly upregulated after lenalidomide treatment (***P = .001).