Cultured CD146⁺ perivascular cells express markers of hematopoietic perivascular niche cells. (A-B) Ex vivo–expanded CD146⁺ perivascular cells purified from fat and FBM similarly express higher levels of mRNA of perivascular cell markers when compared with MSCs and CD146⁻ cells (n = 2 donors for each tissue). (C-N) Fat and FBM-derived CD146⁺ perivascular cells similarly and almost exclusively express (C-F) nestin and (G-J) CXCL12 in culture compared with CD146⁻ cells. (K-N) No difference in Lep-R expression was observed between CD146⁺ and CD146⁻ cells from either fat and FBM (original magnification, ×20). mRNA, messenger RNA.