The heptad signature is associated with distinct molecular subtypes of AML. (A) Multivariable analysis for OS and EFS for different variables. (B) The cytogenetically normal AML samples were further subdivided into LMR and HMR groups and survival recalculated based on the Heptad-sig. The panel to the left shows Kaplan-Meier plots for OS with the HMR group as a collective. The panel to the right shows survival for individual combinations that comprise the HMR group. (C) Distribution of heptad gene expression in NPM1wt and NPM1mut AML in 2 independent CN-AML cohorts. The box plots visualize the distribution of gene expression levels within these groups. Significance between the NPM1wt and NPM1mut groups was assessed using the Mann-Whitney U test. *P < .05; **P < .01; ***P < .001; and ****P < .0001. Black boxes, NPM1 wt cohort; green boxes, NPM1 mutant.