Hdac1 knock-down primes the expansion of Gr-1⁺/c-Kit⁺ cells in vivo. Lin⁻ cells derived from mCG^PR/PR transgenic mice were transduced with the indicated vectors, and sorted GFP⁺ cells were injected into lethally irradiated mice. Mice were euthanized 30 days after transplantation, and their phenotype was analyzed. (A) A representative dot plot showing the levels of the indicated differentiation markers in bone marrow cells. Insets show the cytologic analysis of the sorted subpopulations (red squares). (B) Plot of the levels of Gr-1⁺/c-Kit⁺ cells in the indicated murine bone marrow samples. (C) Mice transplanted with the indicated cells were injected with BrdU. In the graph, the levels of BrdU incorporation are shown. (D) The indicated Gr-1⁺/c-Kit⁺ cells were plated in methylcellulose medium and were scored for colony-forming ability. In the graph, the mean and standard deviation of 3 independent experiments are shown. (E) Morphologic analysis of the indicated Gr-1⁺/c-Kit⁺ cells, harvested after plating in methylcellulose medium. Left panels, representative cytospins; right panel, percentage of mature and immature cells, original magnification ×1000, May Grünwald-Giemsa staining, Olympus BX51. Statistical analysis was performed with the Fisher exact test.