NK1R signaling increases the capacity of BMDCs to elicit type 1 immunity. (A-B) Cytokine secretion (analyzed by ELISA) of splenic (A) CD4 or (B) CD8 T cells obtained from C57BL/6 mice 7 days after footpad immunization (1 dose) with untreated DCs, NK1R-DCs, Ag-DCs, or Ag-NK1R-DCs. (C) Analysis of Ag-specific CTL function by in vivo killing assays, 7 days after footpad immunization (1 dose) of C57BL/6 mice with untreated DCs, NK1R-DCs, Ag-DCs or Ag-NK1R-DCs. (A-B) Means ± 1 SD of triplicates from 1 of 3 experiments is shown. (C) Means ± 1 SD from 6 mice per experimental group are shown.