T-Rapa cell recipients have immune reconstitution of a mixed Th2 and Th1 cytokine phenotype. (A) Percentage of CD4⁺ T cells expressing GATA-3, T-bet, and FoxP3 as measured by intracellular flow cytometry at day +14 posttransplant (just before T-Rapa cell DLI) and at 1 month and 3 months post-HCT (comparisons are 1 month vs day 14 and 3 months vs day 14; **P < .001; *P < .05). Intrapatient ratio of GATA-3 to T-bet expressing CD4⁺ T cells is shown for each time point. (B) At 1, 2, 4, and 8 weeks post-HCT, peripheral blood mononuclear cells were costimulated, and the 24-hour supernatant was tested for cytokine content by Luminex (mean ± standard error of the mean; between n = 32 and n = 34 evaluated for each paired analysis; comparisons are week 4 vs week 2 and week 8 vs week 2; **P < .001; *P < .01).