Inhibition of IL-6–induced hypoferremia and anemia by NOX-H94 treatment in cynomolgus monkeys. (A) Treatment with IL-6 induced a nonsustained hypoferremia. The serum iron decrease was prevented by 10 mg/kg NOX-H94 (vehicle A, 5% glucose; vehicle B, 1% autologous heat-inactivated serum). In the control group without IL-6, NOX-H94 treatment induced a temporal hyperferremia (n = 3 per group, mean ± SEM). Statistical analysis was performed with the unpaired t test comparing the different time points of each group with the respective predose values. The dotted line indicates the mean predose serum iron concentration. (B) Repeated subcutaneous injections of human IL-6 (0.3 µg/kg, daily) reduced hemoglobin concentration (Hb), erythrocyte count (RBC), reticulocyte hemoglobin content (Chr), hematocrit, and mean corpuscular hemoglobin concentration (MCHC). This effect was ameliorated in NOX-H94–treated (10 mg/kg, daily) animals (mean ± SEM, n = 4 per group, individual levels describe the difference compared with predose levels). Statistical analysis was performed with Dunnett’s multiple comparison test. *P < .05; **P < .01.