PIP5KIγ colocalizes with talin in primary megakaryocytes. (A) Hematopoietic progenitor cells were differentiated into megakaryocytes and infected with a retrovirus that directed the expression of the GFP-p90 PIP5KIγ fusion protein. Shown are confocal micrographs demonstrating the intracellular distribution of (top) GFP-PIP5KIγ and (middle) talin. The bottom panel is a merged image of GFP-PIP5KIγ and talin suggesting that PIP5KIγ colocalizes with talin in primary megakaryocytes. (B) Megakaryocytes expressing either p87 or p90 GFP-PIP5KIγ fusion protein were stained with mouse anti-talin and rabbit anti-GFP antibodies followed by both secondary immunogold particles, conjugated anti-rabbit IgG (10 nm), and anti-mouse IgG (5 nm). The small gold particles demonstrate the localization of talin (blue circles), and the big gold particles (red squares) demonstrate the distribution of the GFP-PIP5KIγ fusion protein. The magnification bar is 100 nm. (C) The frequency of talin colocalizing with p87 or p90 PIP5KIγ.