PRC1 paralog family members show lack of redundancy in human CD34⁺ cord blood (CB) cells. (A) Schematic representation of the lentiviral pLKO.1 GFP vector. (B) FACS analysis of transduced CD34⁺ CB cells at day 2 after transduction. (C) qPCR analyses of a representative experiment showing knockdown efficiency of the various shRNAs. SCR and the specified knockdown are indicated with “S” and “K,” respectively. Error bars indicate standard deviation of triplicate measurements. (D) Cumulative expansion of GFP⁺ cells in bone marrow stromal cocultures on MS5 cells. (E) Cumulative expansion of GFP⁺ cells in liquid culture assays. (F) The effects of knockdown of PRC1 members on progenitor frequencies (F) and subtype (G) as determined by CFC assays. cum., cumulative.