Increased random migration of PID-transduced HSPC in response to SDF-1α. (A-B) Time-lapse microscopy of GFP+ cells from PID or MIEG3-transduced LSK cells on a fibronectin-coated coverslip in the presence of an SDF-1α gradient. (A) Paths followed by individual cells in 1 hour. Representative fields (325 × 325 μm); data shown represents 1 of 2 independent experiments. (B) Total displacement of migration in 1 hour. Data represent mean ± SEM (N = 9-19 cells analyzed per group). **P < .01. (C-D) Chemokinesis of LSK measured after 2 hours in a transwell chamber assay as a result of (C) uniform concentration of SDF-1α or (D) 2% bovine serum albumin alone. Data represent the mean ± SEM, of the percentage of migrated cells (in bottom chamber) normalized to input (total number of cells plated to top chamber), performed in triplicate (N = 3 independent experiments). *P < .05; **P < .01.