The C-terminal domain of CgA is crucial for its anti-angiogenic activity. Effect of CgA fragments lacking the C-terminal region (CgA1-373, 1-400, and 1-409; top panels) or corresponding to the C-terminal region (CgA410-436, pGlu-serpinin, and CgA410-439; bottom panels) on bFGF and VEGF-induced angiogenesis in the RAR assay. Bars represent the number of capillary-like structures emerging from the aorta rings expressed as percentage of the untreated control (mean ± SD; *P < .05, **P < .01, ***P < .001; 2-tailed t test).