Full-length CgA inhibits spontaneous, bFGF and VEGF-induced angiogenesis. (A) Effect of the indicated doses of CgA1-439 and suramin on angiogenesis in the CAM assay. (Left) Stereomicroscope photographs of vessels recruited by the 6-mm (diameter) filter-paper disks (arrows; bar 1 mm). (Right) Quantitative evaluation of angiogenesis obtained with the indicated number of eggs (n). Blood vessels recruited by the disk, but not vessels that did not touch the filter, were counted in a blind manner by 2 observers. Circles correspond to vessel recruited by half-disk in each egg (box plots with median, interquartile, and 5%-95% values; **P < .01, ***P < .001; 2-tailed t test). (B) RAR assay. Effect of CgA1-439 on spontaneous, bFGF, or VEGF-induced angiogenesis. (Top panels) Bars represent the number of capillary-like structures emerging from the aorta rings treated as indicated, expressed as percentage of the untreated control (mean ± SD). The number of rings used is indicated in each panel (n). (Bottom panels) Microscopy photographs of aorta rings (white arrowheads) showing capillary-like structures (black arrows, 5× magnification, bar 150 μm; *P < .05, **P < .01, ***P < .001; 2-tailed t test).