Agarose gel-based conventional AS-PCR assay for detection of MYD88 L265P. (A) MYD88 L265P AS-PCR primer design. The reverse primers with an internal mismatch in the third position from the 3′-end and the common forward primer are indicated by arrows. (B) Sensitivity of the conventional AS-PCR assay was established by serial dilutions of DNA from OCI-LY3 against OCI-LY19 cells. The PCR products (159 bp) for this assay were separated on a 2% agarose gel as indicated by arrows. The mutant MYD88 L265P allele was detected to a dilution of 0.1%. MW, molecular weight.