Figure 1
Viral vector-loaded B cells are more potent than DCs for boosting CD8+ T-cell immunity. C57BL/6 mice were primed IP with 2 × 107 pfu of Vac-GP33 and boosted with 3 × 106 B/VSV-GP33 by i.v. injection or DC/VSV-GP33 via either IV or footpad administration at a 14-day (A) or 30-day (B) interval. Five days post-boost, the frequency of GP33-specific T cells in blood were enumerated by detection of intracellular IFNγ production following in vitro stimulation with the peptide. *Significantly higher CD8+ T-cell response compared with all other groups (P < .01). Data are representative of duplicate experiments using 5 mice per group, per experiment.
