Figure 5.
MegP transcriptome was distinct from those of other HSPCs. (A) Gene expression profiling of BM-derived HSPCs analyzed by microarray. Unsupervised hierarchical clustering analysis on differentially expressed genes among analyzed populations is shown in the dendrogram together with a heat map representing gene expression level by color (top). The thick pink line demarcates 5 distinct clusters (C1-C5). C2, C3, and C5 genes were upregulated in lineage-committed HPCs (GMPs, MegPs, and MEPs, respectively). The biological meanings of C1-C5 cluster genes were investigated using the Database for Annotation, Visualization and Integrated Discovery tool, and results are listed in the table (bottom). (B-D) Gene expression patterns of hematopoietic lineage-affiliated gene sets are shown in heat map displays. MegPs predominantly expressed megakaryocyte-specific genes. The following two gene sets were obtained from the Molecular Signatures Database: (i) REACTOME PLATELET ACTIVATION SIGNALING AND AGGREGATION and (ii) REACTOME PLATELET ADHESION TO EXPOSED COLLAGEN. Ery, erythroid.

MegP transcriptome was distinct from those of other HSPCs. (A) Gene expression profiling of BM-derived HSPCs analyzed by microarray. Unsupervised hierarchical clustering analysis on differentially expressed genes among analyzed populations is shown in the dendrogram together with a heat map representing gene expression level by color (top). The thick pink line demarcates 5 distinct clusters (C1-C5). C2, C3, and C5 genes were upregulated in lineage-committed HPCs (GMPs, MegPs, and MEPs, respectively). The biological meanings of C1-C5 cluster genes were investigated using the Database for Annotation, Visualization and Integrated Discovery tool, and results are listed in the table (bottom). (B-D) Gene expression patterns of hematopoietic lineage-affiliated gene sets are shown in heat map displays. MegPs predominantly expressed megakaryocyte-specific genes. The following two gene sets were obtained from the Molecular Signatures Database: (i) REACTOME PLATELET ACTIVATION SIGNALING AND AGGREGATION and (ii) REACTOME PLATELET ADHESION TO EXPOSED COLLAGEN. Ery, erythroid.

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