CD41⁺CMPs show strong platelet-producing ability in a xenotransplantation model. (A) Experimental schema of xenotransplantation. CB-derived HSPCs were injected into BRGSK^Wv/Wv mice via femur. After 3 weeks, chimerism of progenies in each lineage (CD33⁺ myeloid cells, CD19⁺ B cells, CD3⁺ T cells, GPA⁺ erythrocytes, CD41⁺ platelets) was analyzed using FACS. For the evaluation of erythrocytes or platelets, the forward-scatter (FSC) detector voltage was magnified, and FSC^low cells (black rectangle) were analyzed. (B) Chimerism of human platelets, erythrocytes, and myeloid and lymphoid cells was determined after transplantation of each progenitor fraction. Engraftment of human myeloid and lymphoid cells was assessed by absolute counts. Bars indicate means ± standard deviations. *P < .05 (1-way analysis of variance followed by Tukey’s HSD test; n = 3). (C) Immunophenotypic changes of BM-derived CD41⁻ CMPs after a 7-day liquid culture. CD41⁻ CMPs gave rise to CD41⁺ CMPs (CD34⁺ CD38⁺ IL-3Rα^dim CD45RA⁻ CD41⁺). Representative FACS plots from 3 independent experiments are shown. SSC, side scatter.