Single-cell gene expression profiling revealed a distinct CMP subpopulation characterized by expression of megakaryocyte-specific genes. Heat map visualization of single-cell gene expression profiling of BM-derived HSPCs. Gene expression levels of individual single-cells from (A) HSC, (B) MEP, and (C) CMP fractions are shown. Genes related to erythroid (Ery), megakaryocyte (Meg), or GM lineage are listed on the left side of the heat map. Annotation for each gene is presented in supplemental Table 1. Representative heat maps from 3 independent samples are shown. (B) Most MEPs expressed erythroid-specific genes (red rectangle) but not megakaryocyte-specific genes. (C) A cluster of cells that exclusively expressed megakaryocyte-specific genes at a high level was observed (green rectangle).