EETosis induces the release of intact cell-free granules through budding and cell lysis. The graphic shows the temporal course of the morphologic changes exhibited by eosinophils undergoing EETosis. Stimuli-elicited NADPH oxidase activation leads to the common process of eosinophil cytolysis, followed by the loss of the typically bilobed nuclei into a single round nuclei. During this time, some eosinophil granules were released extracellularly as PM-enveloped structures. Thereafter, nuclei disintegrated to form intracellular DNA nets. Subsequently, the eosinophils’ PMs rupture, releasing both a weblike chromatin structure and free eosinophil granules. EETosis-mediated cytolysis leads to the extracellular liberation of intact granules, some of which retained their capacity for secretory responses.