EETosis developing within 120 minutes in an NADPH oxidase-dependent manner. Eosinophils (in 0.1% BSA and CYTOX containing RPMI medium) incubated with (A) 1 mg/mL immobilized IgG and (B) 2 μM A23187 with the indicated stimuli concentrations induced temporal EETosis cytolysis (extracellular CYTOX intensity) attributable to DPI-inhibitable NADPH oxidase activity. For (A), data are means ± SD, from 3 different donors, and for (B), data are means ± SD from 4 different donors.