Gene expression profile of cultured EPCR⁺population is enriched with HSC-related genes. (A) Scatter plot of global mRNA profiling showing upregulated (red) and downregulated (blue) genes in EPCR⁺ versus EPCR^Rest (EPCR^– and EPCR^low) populations (selection criteria: P ≤ .01, mean expression ≥1 RPKM; ≥ 2-fold difference). (B) GSEA plot showing enrichment for HSC-related genes in the EPCR⁺ population. Gene expression profiles of uncultured purified human HSCs derived from CB and fetal liver (FL) from 2 independent data sets were used for the GSEA analysis. All comparisons were significant. (C) Venn diagram showing the number of common and distinct upregulated genes in the EPCR⁺ population in CD34⁺CD38^low/–CD90⁺ FL cells and in CD34⁺CD38^– (FL and CB) cells. mRNA (D) and protein (E) levels of known HSC surface markers in various CB cell populations. GPR56, G protein-coupled receptor 56; NES, normalized enrichment score.