Expanded EPCR⁺progeny retains most of the HSPC activity. (A) Schematic representation of EPCR sorting and transplantation strategy to characterize the progeny of the EPCR⁺ population. (B) FACS profiles showing expression of the indicated markers on cultured EPCR subsets. (C) Fold expansion of the indicated HSPC populations from day 3 to day 10 of the culture (mean ± SD, 3 technical replicates). Differences between conditions are statistically significant unless labeled as not significant (ns; Mann-Whitney U test, 1-sided). (D) Engraftment levels of EPCR subset progeny in NSG mice at day 10 of the culture. Horizontal bars indicate median values (n = 6 to 7 mice per condition, technical replicates). *P < .05 (Mann-Whitney U test, 1-sided).